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6:19 am December 11, 2011
| Daniel Bandi
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| New Member | posts 2 | |
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We commonly use a conventional fiberglass type brush and conventional fingerprinting powders to develop latent prints. The question has arisen if the same brush is used from crime scene to crime scene can this potentially deposit DNA from one scene to another? I am looking for any studies I can be pointed to that indicate the potential for cross contamination of DNA from using the same latent print brush and / or powder. Thanks!
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10:45 am December 12, 2011
| Karen Green
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Daniel
Here is an article in Volume 50 Issue 6 of the Journal of Forensic Sciences. I haven't read beyond the abstract, but here's a link: http://www.astm.org/DIGITAL_LI…..004430.htm
As a DNA scientist and crime scene investigator I understand that while encouraged, it is not always practical to use disposable brushes. At a minimum I recommend aliquoting the fingerprint powder into a smaller container (to be disposed of at the end of the scene.)
Karen
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12:07 pm December 12, 2011
| Daniel Bandi
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Karen,
Thank you. It certainly reinforces how I have been trained. Take Care.
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12:28 pm December 12, 2011
| TAdair
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I think it's pretty much a non-starter issue. Get in the practice of taking your DNA samples (swabbings) prior to powdering and you'll be fine. Tom A.
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2:25 pm December 12, 2011
| Karen Green
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| Member | posts 6 | |
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I certainly try to collect DNA samples prior to the introduction of any outside reagents but often that is not possible when collecting touch DNA samples. Certain areas may have to be processed for prints first to make sure I’m not destroying anything while collecting my DNA swabs. I may also collect DNA swabs from areas that show ridge detail after processing but are insufficient for comparison.
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8:14 am December 22, 2011
| EToomer
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In our agency, we have gone completely to disposable brushes and powders. We still use fiberglass brushes, since they are better than the "off the shelf" disposable brushes, but we throw them away after a single use. Additionally, our lab (USACIL) has had luck getting both Touch DNA and latent print results from lifts using traditional fingerprint collection methods.
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